During isolation of DNA, RNase treatment is performed to eliminate contaminating RNA. Because RNase activity is robust and resistant to denaturation, commercial preparations of DNA contain high levels of residual RNase activity, even after re-purification. Preparing DNA solutions in DEPC-treated water will not inactivate RNase already present in the DNA. Similarly, commercial RNase inhibitor additives are usually poorly effective, expensive, and interfere in downstream applications. Biotium has developed a unique proprietary method that irreversibly inactivates RNases in purified DNA. The resulting DNA solution is free of excess protein or other chemicals that may interfere with downstream applications. Our RNase-free Calf Thymus DNA solution undergoes stringent quality control testing to confirm the product is 100% free of RNase activity. The solution is provided as 1 mg/mL calf thymus DNA in TE. Store the product at 4⁰C.